And fans on social media were quick to praise Messi.
One said: "CR7 makes a Hat-Trick.. Messi wanting to make forget that by throwing a Panenka."
Another explained: "The Panenka of Messi is in the top of the best I've seen."
It means Barcelona are on the verge of making it into the quarter-finals of the Champions League.
Lionel Messi netted a perfect penalty against Lyon (Image: GETTY)
Lionel Messi scored an outrageous penalty (Image: GETTY)
The draw takes place on Friday morning at 11am.
Ronaldo scored his eighth European hat-trick last night to draw level with Messi as he took him his first match ball as a Juventus player.
But Messi was in no mood to be outdone tonight at the Nou Camp.
His Panenka penalty was a stark reminder why he is regarded by most as the best player to ever take to a football pitch.
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Lionel Messi and Philippe Coutinho were both on target for Barcelona tonight (Image: GETTY)